Part:BBa_K3037000:Design

pOCC97 plasmid backbone for expression (optimized)

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 5283
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  Illegal NotI site found at 9
  Illegal NotI site found at 5289
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 5283
  Illegal BglII site found at 5169
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found at 5283
  Illegal suffix found at 2
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found at 5283
  Plasmid lacks a suffix.
  Illegal XbaI site found at 5298
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  Illegal NgoMIV site found at 342
  Illegal NgoMIV site found at 3389
  Illegal NgoMIV site found at 3549
  Illegal NgoMIV site found at 5137
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal SapI.rc site found at 2468
  

Design Notes

This BioBrick was site mutated to take out a XbaI restriction site